The Relationship of the Age of Rabbit Erythrocytes to the Effects of Inosine on their Osmotic Resistance12

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Abstract
Glycine-2-C14 was administered to a series of rabbits and, at intervals of 4, 8, 46 and 60 days after injection, the washed erythrocytes from one of the rabbits were incubated for 2 hours at 37[degree]C in isotonic sodium phosphate buffer (pH 7.3) alone or with 15 jumoles of inosine per ml of suspension. By subjecting these erythrocytes to osmotic stress in hypotonic buffer solutions and determining the specific activity of the hemin isolated from the lysed cells it was demonstrated that: (1) Young erythrocytes are more resistant to hypotonic lysis than the average of the whole cell population, and (2) incubation with inosine increases resistance to osmotic stress of young erythrocytes much more than that of the average of the total cell population.