Enzyme‐linked immunosorbent assay for detection of antibodies to the unmodified beta‐lactam ring

Publisher Website
Google Scholar
Abstract
Dose‐ and pH‐ dependent carbodiimide‐mediated coupling of Penicillin‐G to polystyrene microtiter‐plates that leaves the beta‐lactam ring unchanged is described. A new ELISA method was developed using Penicillin‐G coated plates. The binding of 3 different monoclonal antibodies as well as human IgG antibodies of the IgG1 and IgG3 subclasses is demonstrated, whereas IgG2, IgG4 and IgE antibodies did not bind. Thus, covalently coupled Penicillin‐G can be used to study the immune‐response to the unchanged β‐lactam ring in patients receiving penicillin therapy. The new method is complementary to hitherto described techniques, which generally only allow detection of antibodies binding to penicilloyl‐groups.