SEC–viscometer detector systems. II. Resolution correction and determination of interdetector volume

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Abstract
The closely related topics of resolution correction and the determination of interdetector volume were examined. In determining the interdetector volume (δ) by searching to superimpose two types of intrinsic viscosity calibration curve (one from narrow standards and one from a broad standard), the underlying equation based upon symmetrical axial dispersion theory was derived. This equation combined with experimental results showed that the local intrinsic viscosity value is very similarly affected by interdetector volume and by band spreading. The result supported the idea of using an effective δ to effect an axial dispersion correction to local intrinsic viscosity data. However, it also increased the difficulty of finding both δ and standard deviation (δ) simultaneously by numerical search. Furthermore, attempts to apply the method to the chromatograms of narrow standards showed inadequate superposition. Following calculation of skewing factors, the superposition problems were attributed to skewing of the chromatograms of the monodisperse polymer standards used. © 1993 John Wiley & Sons, Inc.