Characterization of two substance P antisera
- 1 January 1982
- journal article
- research article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 114 (1) , 53-57
- https://doi.org/10.1111/j.1748-1716.1982.tb06951.x
Abstract
The cross reactivity of two currently used substance P (Sp) antisera (K 16 and K 25) with various analogues of SP, partial sequences of SP and other naturally occurring peptides were tested in a radioimmunoassay system. Both antisera were found to be C-terminal directed, this tendency being most pronounced for antiserum K16. The capacity of antiserum K 25 to bind various SP-related peptides correlated to the previously described biological activity of these peptides on the isolated guinea pig ileum. The results thus indicate that this antiserum binds to a biologically active part of the SP-molecule.Keywords
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