Comparison of the electrophoretic method with the sedimentation method for the analysis of DNA strand breaks.
Open Access
- 1 January 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Radiation Research
- Vol. 23 (3) , 385-398
- https://doi.org/10.1269/jrr.23.385
Abstract
Application of electrophoresis to the analysis of DNA strand breaks was studied comparing with the sedimentation analysis. A BRL gel electrophoresis system (Type V16) was used for this study. Calf thymus DNA (1 mg/ml) irradiated with 60Co gamma-rays in SSC solution was applied to both the electrophoretic analysis and the sedimentation analysis. Lamda phage DNA and its fragments were employed as the standard size molecules. In a range from 1 k base pairs to 6 k base pairs in length for double stranded DNA or from 2 k bases to 12 k bases for single stranded DNA, the calculated average molecular weight from the electrophoresis coincided with that from the sedimentation. Number of single strand breaks and double strand breaks were 1.34 × 1011 breaks/mg/rad (G = 0.215) and 0.48 × 105 breaks/mg/rad2, respectively.This publication has 14 references indexed in Scilit:
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