Some Growth Factors for Hemolytic Streptococci

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Abstract
By the use of an alkali-treated medium it has been shown that a number of hemolytic streptococci require riboflavin, pantothenic acid, and a suitable reducing compound. No reducing substance was found to be specific, for ascorbic acid, glutathione, thioglycol-lic acid, or reduced iron were each found to be effective for Streptococcus epidemicus. Reduced iron was the most effective reducing substance tried. Species from the Lancefield Groups A, B, C and D were shown to behave similarly to S. epidemicus. For most of the organisms examined, sodium sulfide was beneficial when added to a medium rendered free of sulfides and alkali-labile sulfhydryl groups. For a few it appeared to be essential. A few organisms were found which required some, but not all, of the alkali-labile factors.