Semicontinuous cultivation of Digitalis lanata cells: Production of β‐methyldigoxin in a 300‐L airlift bioreactor
- 5 August 1989
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 34 (4) , 502-508
- https://doi.org/10.1002/bit.260340410
Abstract
Selected Digitalis lanata cell lines cultivated in 1-L shake flasks or 20-L airlift bioreactors converted β-methyldigitoxin into β-methyldigoxin with almost no side reactions. This biotransformation process was optimized with regard to substrate supply and culture medium composition, and was then scaled up to a volume of 210 L using a 300-L airlift bioreactor. A semicontinuous process was developed in which 513.3 g β-methyldigoxin were produced after 89 days of cultivation.This publication has 16 references indexed in Scilit:
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