Senescence: Action of Auxin and Kinetin in Control of RNA and Protein Synthesis in Subcellular Fractions of Bean Endocarp

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Abstract
A comparative study was made of the effects of auxin ([alpha]-naphthalene acetic acid), kinetin (6-furfurylaminopurine) and a mixture of auxin and kinetin applied in vivo on synthesis of RNA and protein and the distribution of such synthesis among the subcellular fractions of sections of endocarp from ''Kentucky Wonder'' pole beans (Phaseolus vulgaris. L.). Auxin caused considerable enhancement of incorporation of labeled precursors into RNA and protein of all subcellular fractions, and induced net synthesis of RNA and protein. That auxin-induced net synthesis of protein is repressed by actinomycing D indicates that auxin acts primarily to stimulate synthesis of RNA, as a result of which synthesis of protein is enhanced. The effect of kinetin alone on synthesis of RNA, or of kinetin on auxin-induced synthesis of RNA was variable, with either stimulation or hinibition observed in different experiments. Kinetin-enhancement of synthesis of both RNA and protein in subcellular fractions also varied, with enhancement of synthesis in 1 or all subcellular fractions among different experiments. The variable effect of kinetin did not seem to be related to the amount of endogenous or added auxin. The mode of action of kinetin is discussed.