The Relation of Ultrastructure to DNA Synthesis in Human Leucocytes

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Abstract
The ultrastructure of activated and deoxyribonucleic-acid (DNA)-synthesizing human lymphocytes in phytohemagglutinin (PHA) cultures and in infectious mononucleosis was studied. A "thick-thin" sectioning technique of combined autoradiography and electron microscopy was employed. Both cell types had very similar ultrastructural organization. Cells in DNA synthesis were observed to have: Increased content of cyto-plasmic "free" ribosomes, Golgi zone elaboration and associated increase in numbers of mitochondria and vesicularity, alteration of chromatin density and distribution and nuclear membrane changes.