A 3' TRUNCATION OF MYC CAUSED BY CHROMOSOMAL TRANSLOCATION IN A HUMAN T-CELL LEUKEMIA INCREASES MESSENGER-RNA STABILITY
- 1 May 1990
- journal article
- research article
- Vol. 5 (5) , 707-711
Abstract
The proto-oncogene MYC is rearranged at its 3'' end in the human T-cell leukemia line Hut 78 as a result of a translocation between the long arms of chromosomes 8 and 2. The nucleotide sequence at the breakpoint shows that the rearranged allele of MYC is truncated 24 nucleotides before the first poly(A)-addition signal. The 3'' truncated MYC lacks a 61 nucleotide AT-rich sequence that has been reported to mediate selective mRNA degradation. We show that the truncation results in prolonged stability of MYC mRNA: the half life of the MYC mRNA in Hut 78, as well as in Rat 1A cells transfected with the truncated allele of MYC is increased by at least 3-fold. Our results document yet another mechanism by which MYC may be rendered pathogenic and dramatize the importance of mRNA stability in the regulation of MYC activity.This publication has 17 references indexed in Scilit:
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