IMMUNE-RESPONSES OF COWS TO A CHEMICALLY MODIFIED ANAPLASMA ANTIGEN

  • 1 January 1978
    • journal article
    • research article
    • Vol. 39  (6) , 925-930
Abstract
Cows were vaccinated at 0 and 28 days with a standard or a chemically modified Anaplasma antigen. The humoral antibody response was measured by the Anaplasma complement-fixation and card agglutination tests. The antibody response to bovine erythrocyte antigens was also evaluated. Peripheral blood leukocytes were collected for an in vitro lymphocyte-transformation test. Both groups of vaccinated cows developed a significant cell-mediated response as measured by lymphocyte-transformation tests. Only the cows given the standard Anaplasma antigen developed Anaplasma antibody and significant titer of bovine erythrocyte antibodies. Vaccinated and control cows were challenge exposed 1 mo. after the 2nd antigen injection. The vaccinated cows developed lower levels of parasitemia than did the control nonvaccinated cows.