Microbial and Hybrid Sensors for Determination of α-Amylase Activity

Abstract
Microbial (maltose induced B. subtilis) and hybrid (B. subtilis and glucoamylase) sensors have been developed for determination of α-amylase activity by measuring the change of respiration rate of microorganisms caused by uptake of splitting products of starch. Up to 3 I.U. bacterial α-amylase a linear relationship between enzyme activity and change of respiration rate has been found with the hybrid sensor.