KINETIC EFFECTS OF SANGIVAMYCIN IN SARCOMA-180 INVITRO

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (5) , 1784-1788
Abstract
The lethal and sublethal effects of sangivamycin (SGM) were studied in [mouse] sarcoma 180 cells in vitro in relation to drug concentration and duration of drug exposure. SGM lethality was dependent on drug concentration and duration of drug exposure. Pronounced effects on cell survival were observed only when SGM exposure was prolonged; with prolonged drug exposure, small increments in SGM concentration resulted in large increases in cell killing. Log-phase cells were more susceptible to the lethal effects of SGM than were early-plateau-phase cells. Measurements of incorporation of [3H]thymidine and [3H]uridine into the acid-insoluble cell fraction demonstrated inhibition of DNA and RNA synthesis by SGM which was also dependent on drug concentration and duration of drug exposure, reflecting the lethality characteristics of SGM. As SGM concentration was increased, DNA synthesis was inhibited more rapidly than was RNA synthesis. Flow cytometry demonstrated a concentration- and time-dependent accumulation of cells in the late S and G2-M region of the DNA histogram. Maximum lethality apparently is obtained by prolongation of SGM exposure. Pharmacokinetic studies may be important for determining regimens which provide such exposure in humans.