Immunological Studies of the Luteinizing Hormone. I. Identification of the Antigenic Components of Sheep Luteinizing Hormone and the Development of a Serologically Specific Antiserum

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Abstract
Hyperimmune antiserum to [National Institutes of Health] NIH luteinizing hormone (LH) after absorption with sheep serum and sheep tissues developed 7 precipitin lines against luteinizing hormone in im-munoelectrophoresis and agar gel diffusion. Consecutive absorption with follicle-stimulating hormone (FSH), pregnant mare''s serum (PMS), human chorionic gonadotropin (HCG), and, finally, thyroid-stimulating hormone (TSH), resulted in removal of specific lines with each absorbing hormone. After the last absorption a single line remained, and the final serum was no longer serologically reactive with any of the hormones studied except the luteinizing hormone.