Chromatographic separation of brain lipids. 2. Ethanolamine-containing phospholipids

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Abstract
Chromatography of mixed rat-brain lipids on an alumina column by a gradient method, in which the water content of a chloroform -methanol solvent was raised from 7 to 12.5% (by vol.), resulted in the elution of a peak containing all the ethanolamine-based phospholipids. The main components were phosphatidylethanolamine and ethanolamine plasmalo-gen, but smaller quantities of sulphatide, lysophosphatidylethanolamine and "excess ester" were also present. Chromatography of this partially purified material on silicic acid, whereby the methanol content of a chloroform-methanol solvent system was gradually raised from 2 to 9% (by vol.), produced a satisfactory separation into the following frac- tions: (a) "excess ester"; (b) a mixture of phosphatidylethanolamine and ethanolamine plasmalogen; (c) a mixture of sulphatide and lysophosphatidylethanolamine. Phosphatidylethanolamine and ethanolamine plasmalogen have been found to be moderately stable when in contact with alumina.