Antibody to human follicle-stimulating hormone: cross-reactivity with three other hormones

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Abstract
Antibodies directed against human follicle-stimulating hormone (FSH) were demonstrated in rabbit serum by neutralization of biological activity. Antibodies that bound FSH-131I were produced in rabbits and guinea pigs by repeated injections of FSH. By 131I immunochemical methods, we found that at least 90% of the FSH-131I-binding antibody failed to distinguish the four human glycoprotein hormones: FSH, luteinizing hormone, chorionic gonadotropin, and thyrotropin, purified as well as endogenous hormone in plasma. Neither growth hormone, adrenocorticotropin, nor a variety of glycoproteins or animal plasmas were able to react with these antibodies.