Overexpression of Gsα in NG108‐15, neuroblastoma X glioma cells: effects on receptor regulation of the stimulatory adenylyl cyclase cascade
- 18 November 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 397 (2-3) , 325-330
- https://doi.org/10.1016/s0014-5793(96)01208-2
Abstract
Neuroblastoma X glioma hybrid, NG108‐15 cells were stably transfected with an epitope tagged variant of Gsα(HAGsα). The introduced HA‐Gsα was able to interact with the IP prostanoid receptor and was able to stimulate adenylyl cyclase activity as measured by an enhanced capacity of membrane extracts to reconstitute NaF‐dependent adenylyl cyclase activity to membranes of S49 lymphoma cyc− cells. Despite this, neither the maximal stimulation nor the potency of agonist ligands at the IP prostanoid, A2 adenosine or secretin receptors was altered substantially compared to the parental cells although the basal adenylyl cyclase activity was increased. These data indicate that cellular levels of Gsα do not limit signal transduction capacity in NG108‐15 cells, whereas enhanced expression of adenylyl cyclase allows greater maximal cAMP generation following receptor activation (MacEwan, D.J., Kim, G.D. and Milligan, G. (1996) Biochem. J. 318, 1033–1039).Keywords
This publication has 14 references indexed in Scilit:
- The stoichiometry of expression of protein components of the stimulatory adenylyl cyclase cascade and the regulation of information transferCellular Signalling, 1996
- Quantification of signalling components and amplification in the β-adrenergic-receptor-adenylate cyclase pathway in isolated adult rat ventricular myocytesBiochemical Journal, 1995
- Overexpression of Gs alpha protein in the hearts of transgenic mice.Journal of Clinical Investigation, 1995
- Equivalent regulation of wild type and an epitope‐tagged variant of Gsα by the IP prostanoid receptor following expression in neuroblastoma × glioma hybrid, NG108‐15, cellsFEBS Letters, 1994
- Enhanced Myocardial Function in Transgenic Mice Overexpressing the β 2 -Adrenergic ReceptorScience, 1994
- Quantitative stoichiometry of the proteins of the stimulatory arm of the adenylyl cyclase cascade in neuroblastoma x glioma hybrid, NG108‐15 cellsEuropean Journal of Biochemistry, 1994
- Specific atrial overexpression of G protein coupled human 1 adrenoceptors in transgenic miceCardiovascular Research, 1993
- Activation of the alpha subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity.The Journal of cell biology, 1992
- β-ADRENERGIC RECEPTOR REGULATION IN THE HEART IN PATHOPHYSIOLOGIC STATES: ABNORMAL ADRENERGIC RESPONSIVENESS IN CARDIAC DISEASEAnnual Review of Physiology, 1991