A soluble deoxyribonucleic acid in the mosquito Aedes aegypti.

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Abstract
A maximum of 41% of the total DNA obtained from homogenates of mosquito larvae was found in the supernatant fraction. This DNA, designated sDNA, was essentially absent in similar fractions prepared from pupae and adult mosquitoes. Different conditions of homogenization and centrifugation gave varying concentrations of sDNA, but consistently high levels were found regardless of procedure. Incubation of larval samples did not change the yield of sDNA. The addition of calcium ion or of adult fractions to larval supernatants decreased their sDNA concentration. sDNA was isolated from larval homogenates and characterized to be a double-stranded molecule of low molecular weight (about 470,000). The intra-cellular localization and physiological significance of sDNA is discussed.