DEVELOPMENT of DNA PROBES SPECIFIC FOR CAMPYLOBACTER JEJUNI1

Publisher Website
Google Scholar
Abstract
DNA probes specific for Campylobacter jejuni have been developed. A genomic library of C. jejuni was constructed and screened with 32P‐labeled genomic DNA from C. jejuni and C. coli by colony hybridization. Several clones that reacted only with C. jejuni but not with C. coli were selected. Two DNA inserts (1.5 kb and 0.8 kb) were isolated from the positive clones. A modified Southern hybridization technique indicated that these two 32P‐labeled DNA inserts were specific for C. jejuni. the 1.5 kb DNA insert was partly sequenced, and a 19 base oligomer probe was selected from the sequences. Southern hybridization indicated that the oligomer probe hybridized with DNA from all seven strains of C. jejuni tested bus did not hybridize with DNA from four strains of C. coli, one strain of C. fetus and C. laridis, or 11 other bacterial species tested.