Enhancement of Polio-RNA Infectivity by Dimethylsulfoxide

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Abstract
Summary Dimethylsulfoxide (DMSO) has been shown to enhance infection by polio-RNA. This enhancement was present for all concentrations of DMSO from 5% to 80%. The enhancement was optimal at 40% DMSO with a 20-minute absorption period at room temperature. A significant percentage of the nucleic acid infectivity was absorbed in the first two minutes. Dimethylsulfoxide enhanced RNA infection to a greater degree than M MgSO4, protamine, his tone, and DEAE-dex-tran when tested under the same conditions. There was no enhancement of intact viral infectivity by either DMSO or DEAE-dextran. Addendum: Recently Koch et at(20) have shown an enhancement of polio-RNA infectivity using Poly-L-Ornithine which appears to be slightly greater than DEAE-dextran. The technical assistance of Mr. Bruce Mann is greatly appreciated.