Change in antigen specificity of cytotoxic T lymphocytes is associated with the rearrangement and expression of a T-cell receptor beta-chain gene.
- 1 June 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (12) , 4441-4445
- https://doi.org/10.1073/pnas.83.12.4441
Abstract
Cloned H-Y-specific murine cytotoxic T lymphocytes, which alter antigen specificity in vitro ("aging"), simultaneously exhibit changes in the T-cell antigen receptor .beta.-chain rearrangements and respective mRNAs expressed. .beta.-chain cDNA clones were isolated from a library prepared from mRNA of aged killer T cells. The sequence of the .beta.-chain variable region element (VAK) was found to be identical with germ-line DNA. Four bases at the .beta.-chain diversity-joining region (D.beta.-J.beta.) junction cannot be explained by known germ-line D.beta. and J.beta. elements. These results illustrate that in T-cell clones altered antigen specificity correlates with a switch in productive .beta.-chain rearrangements of the T-cell receptor. When tested for its expression under physiological conditions, significant levels of VAK mRNA were found in normal lymphocyte populations.This publication has 33 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- T-Cell Receptor β-Chain Expression: Dependence on Relatively Few Variable Region GenesScience, 1985
- Rearranged β t cell receptor genes in a helper t cell clone specific for lysozyme: No correlation between Vβ and MHC restrictionCell, 1985
- Structure, expression and divergence of T-cell receptor β-chain variable regionsNature, 1984
- Genomic organization and sequence of T-cell receptor β-chain constant- and joining-region genesNature, 1984
- The T cell receptor β chain genes are located on chromosome 6 in mice and chromosome 7 in humansCell, 1984
- The mouse T cell receptor: Structural heterogeneity of molecules of normal T cells defined by XenoantiserumCell, 1983
- Somatic generation of antibody diversityNature, 1983
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- H-2 compatability requirement for T-cell-mediated lysis of target cells infected with lymphocytic choriomeningitis virus. Different cytotoxic T-cell specificities are associated with structures coded for in H-2K or H-2D;.The Journal of Experimental Medicine, 1975