Thermal denaturation of sheared and unsheared chromatin by absorption and circular dichroism measurements
Open Access
- 1 August 1976
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 3 (8) , 1875-1882
- https://doi.org/10.1093/nar/3.8.1875
Abstract
Thermal denaturation of chromatin is observed by simultaneously monitoring absorption and circular dichroism at 276 nm as functions of temperature. Either observation indicates that sheared chromatin shows less thermal stability than native chromatin. The temperature-dependent ellipticities at 276 nm of these chromatins show features not seen in the absorption curves: the ellipticity of unsheared chromatin increases with temperature, while this increase is abolished or greatly reduced in the same chromatin after shearing. After its first thermal transition (prior to the helix-coil transition) the unsheared chromatin achieves the same ellipticity as sheared chromatin.Keywords
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