StainingToxoplasma GondiiWith Fluorescein-Labeled Antibody: III. The Reaction in Frozen and Paraffin Sections

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Abstract
A procedure is described for staining Toxoplasma gondii in paraffin sections of tissue. Small pieces of tissue are fixed in an absolute ethanol-glacial acetic acid mixture (191) for 1 to 7 days. They are then embedded in paraffin and sections are cut in the usual manner. The paraffin is removed and the sections are stained with specific, fluorescent antibody. Toxoplasma organisms were readily detected by this procedure in tissues from infected mice. Their morphology is recognizable, and the specificity of the stain aids in identification when the organisms occur individually or in small groups.

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