Progress and prospects: naked DNA gene transfer and therapy
Top Cited Papers
- 1 March 2003
- journal article
- review article
- Published by Springer Nature in Gene Therapy
- Vol. 10 (6) , 453-458
- https://doi.org/10.1038/sj.gt.3301983
Abstract
Increases in efficiency have made naked DNA gene transfer a viable method for gene therapy. Intravascular delivery results in effective gene delivery to liver and muscle, and provides in vivo transfection methods for basic and applied gene therapy and antisense strategies with oligonucleotides and small interfering RNA (siRNA). Delivery via the tail vein in rodents provides an especially simple and effective means for in vivo gene transfer. Electroporation methods significantly enhance direct injection of naked DNA for genetic immunization. The availability of plasmid DNA expression vectors that enable sustained high level expression, allows for the development of gene therapies based on the delivery of naked plasmid DNA.Keywords
This publication has 24 references indexed in Scilit:
- RNA interference in adult miceNature, 2002
- CpG-Depleted Plasmid DNA Vectors with Enhanced Safety and Long-Term Gene Expression in VivoMolecular Therapy, 2002
- High‐level expression of naked DNA delivered to rat liver via tail vein injectionThe Journal of Gene Medicine, 2002
- Kidney-Targeted Naked DNA Transfer by Retrograde Renal Vein Injection in RatsHuman Gene Therapy, 2002
- High and Sustained Transgene Expression in Vivo from Plasmid Vectors Containing a Hybrid Ubiquitin PromoterMolecular Therapy, 2001
- Now I know my CpGsTrends in Microbiology, 2001
- Efficient Expression of Naked DNA Delivered Intraarterially to Limb Muscles of Nonhuman PrimatesHuman Gene Therapy, 2001
- Linear DNAs Concatemerize in Vivo and Result in Sustained Transgene Expression in Mouse LiverMolecular Therapy, 2001
- Cutaneous Transfection and Immune Responses to Intradermal Nucleic Acid Vaccination Are Significantly Enhanced by in Vivo ElectropermeabilizationMolecular Therapy, 2001
- Inclusion of the Hepatic Locus Control Region, an Intron, and Untranslated Region Increases and Stabilizes Hepatic Factor IX Gene Expression in Vivo but Not in VitroMolecular Therapy, 2000