Suppressor cell induction in vitro. I. Kinetics of induction of antigen‐specific suppressor cells

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Abstract
The induction of antigen‐specific suppressor cells in vitro, using high concentrations (100 μg/ml) of keyhole limpet hemocyanin (KLH) in Marbrook flasks is described. Spleen and cortisone‐resistant thymocytes were the richest source of suppressor cell precursors, compared to lymph node cells, peripheral blood lymphocytes or thoracic duct lymphocytes. Suppressor cells induced with KLH only suppressed KLH‐reactive helper cells, and not B cells or helper cells of other specificity. The suppressor cells were T cells, as judged by their sensitivity to anti‐Thy‐1.2, heterologous anti‐T, but not anti‐B antisera.