Suppressor cell induction in vitro. II. Cellular requirements of suppressor cell induction

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Abstract
The cellular requirements for the induction of antigen‐specific suppressor cells (SC) in vitro were studied. Macrophage depletion, by either nylon wool or polystyrene columns or iron (and magnet) treatment or a combination of methods, did not diminish SC induction, in contrast to its effect on helper cell induction. The requirement for T‐T interaction in SC induction using splenic T cells was studied by the use of adult thymectomy and antilymphocyte serum, which selectively deplete short‐lived and recirculating T cells respectively. Since suppressor cell induction was markedly diminished, or abolished by either procedure, but reconstituted by mixing these 2 populations, it was concluded that there was T‐T interaction in the development of SC, just as there is in the induction of other T cell reactions, such as the graft‐versus‐host response, the T killer cell response, the helper cell response and, tentatively, delayed hypersensitivity. By the use of double‐chamber tissue culture flasks, with the 2 cell populations separated by a cell impermeable membrane, it was found that T‐T interaction does not require cell contact and is thus mediated by factor(s). The direct precursor of antigen‐specific suppressor cells is resistant to adult thymectomy but sensitive to anti‐lymphocyte serum.

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