Isolation of Higher Molecular Weight DNA from Bacillus Cereus T Using Proteinase K
- 1 January 1974
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 4 (6) , 473-488
- https://doi.org/10.1080/00327487408061549
Abstract
A method is described for the isolation of deoxyribonucleic acid (DNA) from Bacillus cereus T. The DNA is released from the cells autolytically in the presence of proteinase K which prevents degradation by nucleases. The method is simple and eliminates many of the previously required steps which cause mechanical shear. The DNA is of high molecular weight and can be efficiently trapped in agar to give DNA agar which is suitable for hybridization studies.Keywords
This publication has 13 references indexed in Scilit:
- Kinetics of ribonucleic acid-deoxyribonucleic acid membrane filter hybridizationBiochemistry, 1973
- Morphogenesis in Sporulating BacilliCRC Critical Reviews in Microbiology, 1972
- Autolysis of Bacillus cereus cell walls and isolation of structural componentsBiochemical Journal, 1971
- Bacterial Spore Outgrowth Its RegulationScience, 1970
- A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membraneJournal of Molecular Biology, 1965
- A Molecular Approach in the Systematics of Higher OrganismsScience, 1964
- AN APPROACH TO THE MEASUREMENT OF GENETIC RELATEDNESS AMONG ORGANISMSProceedings of the National Academy of Sciences, 1963
- A GENERAL METHOD FOR THE ISOLATION OF RNA COMPLEMENTARY TO DNAProceedings of the National Academy of Sciences, 1962
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961
- Bacterial Protoplasts from Bacillus Species by the Action of Autolytic EnzymesNature, 1957