Synthesis of125I Monolabelled 3, 5, 3′-Triiodothyronine and Thyroxine of Maximum Specific Activity for Radioimmunoassay
- 1 January 1973
- journal article
- other
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 32 (4) , 357-360
- https://doi.org/10.3109/00365517309084359
Abstract
A chloramine T technique for the synthesis of monoiodinated 3,5,3′-125I-T3 and 125I-T4 is described using diiodothyronine and triiodothyronine as 125I-acceptors. Specific activities in the order of 3000 mCi/mg T3 are routinely obtained, i.e. approaching the theoretical maximum value. With these high specific activity products, which are at least 10 times higher than any previously described, it becomes possible to determine free T3 (and T4) directly in ultrafiltrates or dialysates of plasma samples.Keywords
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