A Ca ++ -Dependent and -Selective Ionophore as Part of the Ca ++ + Mg ++ -Dependent Adenosinetriphosphatase of Sarcoplasmic Reticulum
- 1 September 1974
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 71 (9) , 3522-3526
- https://doi.org/10.1073/pnas.71.9.3522
Abstract
Solubilized Ca(++) + Mg(++)-dependent adenosinetriphosphatase (EC 3.6.1.3; ATP diphosphohydrolase) from sarcoplasmic reticulum increased bimolecular lipid membrane (oxidized cholesterol) conductance several hundred-fold. The relative conductance change and the relative permeability elicited by this material has the following sequence: Ba(++) > Ca(++) > Sr(++) > Mg(++) > Mn(++) > Zn(++), Na(+), K(+), Cs(+), Li(+), and Rb(+). Zn(++) and Na(+) strongly inhibit the increase in Ca(++) conductance obtained with solubilized Ca(++) + Mg(++)-dependent adenosinetriphosphatase. The Ca(++)-ionophore is an integral part of the Ca(++) + Mg(++)-dependent adenosinetriphosphatase enzyme and may function as a Ca(++)-carrier in the overall Ca(++)-pump of sarcoplasmic reticulum.Keywords
This publication has 11 references indexed in Scilit:
- Reconstitution of a Calcium Pump Using Defined Membrane ComponentsProceedings of the National Academy of Sciences, 1974
- Surface particles of sarcoplasmic reticulum membranes. Structural features of the adenosine triphosphatase.1974
- Isolation of a High Affinity Calcium-binding Protein from Sarcoplasmic ReticulumJournal of Biological Chemistry, 1974
- STRUCTURAL COMPONENTS OF THE SARCOPLASMIC RETICULUM MEMBRANE*Annals of the New York Academy of Sciences, 1974
- Na + -Selective Ionophoric Material Derived from Electric Organ and Kidney MembranesProceedings of the National Academy of Sciences, 1973
- Reconstitution of a calcium pump with phospholipids and a purified Ca ++ - adenosine triphosphatase from sacroplasmic reticulum.1972
- Ion Transport Through Excitability-Inducing Material (EIM) Channels in Lipid Bilayer MembranesThe Journal of general physiology, 1972
- Isolation of a Calcium-Sequestering Protein from Sarcoplasmic ReticulumProceedings of the National Academy of Sciences, 1971
- Purification and Properties of an Adenosine Triphosphatase from Sarcoplasmic ReticulumJournal of Biological Chemistry, 1970
- POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANESThe Journal of general physiology, 1943