The Detoxification by Acetylation of Soluble Antigens From Shigella dysenteriae (Shiga) and E. typhosa

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Abstract
The toxicity and antigenicity of series of acetylated derivatives of the polysaccharide, or the polysac-charide-protein complex, of Shigella dysenteriae and of Eberthella typhosa (Army strain 58) were investigated. The water-soluble antigens were less toxic after acetylation, as detd. from the loss of weight in mice, some being 60 times less than before acetylation. Mice given 3 subcut. or intraperit. injns. of 0.05 mg. of the acetylated fractions showed active protection against infection with live Shiga organisms. The protection increased with acetylation. Significant detoxification with the protein-polysaccharide complex of E. typhosa was also obtained on acetylation, the order of detoxification being about the same as for the Shiga antigen.