The preparation and properties of a specific polysaccharide from Bact. typhosum Ty2

Publisher Website
Google Scholar
Abstract
A method for the extraction and purification of an O-specific polysaccharide from the bacterial cells of isolated antigenic complex of Bact. typhosum Ty2 is described. The poly- [image] saccharide is dextrorotatory, [image]; it contains 0.2-0.4% N, and 0.4-0.9% P, and yields 83% of reducing sugars on acidic hydrolysis. The polysaccharide appears to be free from ketose, pentose and uronic acid residues but yields 40% d-glucose, 21% d-mannose and 17% d-galactose on hydrolysis. The specific substance precipitates to a high dilution with Bact. typhosum O-antiserum but not with Vi-antiserum. Elimination of the 3% of acetyl groups in the molecule does not appear to influence the limit of precipitation with O-antiserum. The polysaccharide has a minimum molecular weight of the order of 10,000, i.e., about 60 anhydrohexose units.