Zn2+ enhances protein tyrosine kinase activity of human platelet membranes
- 1 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 235 (1-2) , 51-56
- https://doi.org/10.1016/0014-5793(88)81232-8
Abstract
In human platelet membranes enhanced tyrosine phosphorylation of certain proteins was observed when Zn2+ instead of Mg2+ or Mn2+ was used as a divalent cation for the kinase reaction. An enhanced level of phosphate incorporation into tyrosine residues occurred into a 68 kDa polypeptide besides the 45 kDa and 105 kDa proteins. Preincubation of platelet membranes with TBR‐IgG showed a concentration‐dependent inhibition of the phosphorylation of the 45, 68 and 105 kDa proteins. Moreover, pp60c‐src, representing the major protein tyrosine kinase activity in platelets, was found to be stimulated by Zn2+. The data, thus, support the assumption that pp60c‐src kinase is responsible for Zn2+ stimulated tyrosine phosphorylation.Keywords
This publication has 29 references indexed in Scilit:
- Tyrosine kinases in normal human blood cellsFEBS Letters, 1986
- Demonstration of cGMP‐dependent protein kinase and cGMP‐dependent phosphorylation in cell‐free extracts of plateletsEuropean Journal of Biochemistry, 1986
- Detection of a tyrosine kinase in human sera and blood cells by pp60src antiserumBiochemical and Biophysical Research Communications, 1986
- Two separate tyrosine protein kinases in human plateletsFEBS Letters, 1985
- Highly specific antibody to Rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules.The Journal of cell biology, 1985
- High tyrosine kinase activity in normal nonproliferating cellsNature, 1983
- Effects of Zn2+ ions on protein phosphorylation in epithelial cell membranesJournal of Cellular Physiology, 1983
- Structure and sequence of the cellular gene homologous to the RSV src gene and the mechanism for generating the transforming virusCell, 1983
- Detection of a novel mammalian protein phosphatase with activity for phosphotyrosineFEBS Letters, 1981
- Identification of a transformation-specific antigen induced by an avian sarcoma virusNature, 1977