Protein Changes in HL60 Leukemia Cells Associated with 5-Aminolevulinic Acid-based Photodynamic Therapy. Early Effects on Endoplasmic Reticulum Chaperones ¶
- 1 May 2007
- journal article
- Published by American Society for Photobiology in Photochemistry and Photobiology
- Vol. 72 (1) , 16-22
- https://doi.org/10.1562/0031-8655(2000)0720016pcihlc2.0.co2
Abstract
Using two‐dimensional electrophoresis we investigated the effect of 5‐aminolevulinic acid (ALA)–based photodynamic therapy (PDT; induction with 1 mM ALA for 4 h followed by blue light dose of 18 J/cm2) on the protein expression in HL60 leukemia cells. ALA‐PDT resulted in extensive qualitative and quantitative changes in the protein pattern of HL60 cell lysates. Of more than 1350 protein spots recognized on the protein maps of ALA‐induced cells, seven proteins were enhanced and 17 suppressed following irradiation. Three of these, calreticulin presursor, p58 microsomal protein (ERp57) and protein disulfide isomerase (p55) have been identified by matrix‐assisted laser desorption and ionization‐mass spectrometry and the pI/molecular weight parameters of the affected proteins were estimated by computer analysis. The findings suggest participation of endoplasmic reticulum Ca2+‐binding chaperones and/or Ca2+ signaling in ALA‐PDT mediated cytotoxicity.Keywords
This publication has 33 references indexed in Scilit:
- Mitochondria and ApoptosisScience, 1998
- Photodynamic Efficiency of Protoporphyrin IX: Comparison of Endogenous Protoporphyrin IX Induced by 5‐Aminolevulinic Acid and Exogenous Porphyrin IXPhotochemistry and Photobiology, 1997
- In-vitro investigation of ALA-induced protopoyphyrin IXJournal of Photochemistry and Photobiology B: Biology, 1996
- CYTOPLASMIC FREE CALCIUM CHANGES AS A TRIGGER MECHANISM IN THE RESPONSE OF CELLS TO PHOTOSENSITIZATIONPhotochemistry and Photobiology, 1993
- Photodynamic Therapy in Oncology: Mechanisms and Clinical UseJNCI Journal of the National Cancer Institute, 1993
- A nonlinear wide‐range immobilized pH gradient for two‐dimensional electrophoresis and its definition in a relevant pH scaleElectrophoresis, 1993
- Regulation of the genes for heme pathway enzymes in erythroid and in non‐erythroid cellsThe International Journal of Cell Cloning, 1990
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970