Expression profiling using cDNA microarrays
- 1 January 1999
- journal article
- review article
- Published by Springer Nature in Nature Genetics
- Vol. 21 (S1) , 10-14
- https://doi.org/10.1038/4434
Abstract
CDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3´ expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent– or radioactively–labelled cDNAs. Here, we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.Keywords
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