PRODUCTION OF POLY-β-HYDROXYBUTYRIC ACID GRANULES IN MICROCOCCUS HALODENITRIFICANS

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Abstract
Refractile granules of poly-β-hydroxybutyric acid are produced by Micrococcus halodenitrificans during the exponential growth phase in a nitrogenous medium. The amount produced varied from about 1% of the salt-free dry weight during vigorous aeration to as much as 28% with less vigorous aeration. If glycerol, pyruvate, or acetate was added to the medium, the polymer level increased to 40–60%. The polymer was not found in a number of other halophilic bacteria.