Macrophage-chemotactic factor-producing T cell hybridomas reactive to phosphorylcholine in I-E-restricted manner

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Abstract
Macrophage‐chemotactic factor (MChF)‐producing T cell hybridomas were established. These hybridomas produced MChF upon the specific antigenic stimulation with phosphorylcholine (PC)‐conjugated protein in the presence of I‐Ed‐positive antigen‐presenting cells. The hybridomas also secreted MChF after co‐culture with concanavalin A in the absence of antigen‐presenting cells. The MChF‐secreting hybridomas were positive for Kd, Dd, Lyt‐1.2 but negative for I‐Ad, Lyt‐2.2. The molecular weight of MChF produced by the hybridomas was 43000 and the factor was focused at pH 6.6‐6.8 in the chromatofocusing procedure. The MChF was protease sensitive but resistant to neuraminidase treatment, and it was stable by heating at 80°C for 15 min. The MChF‐producing hybridomas established in this study did not produce macrophage migration inhibitory factor (MIF), macrophage‐activating factor (MAF) or interleukin 2, suggesting molecular nonidentity between MChF and these lymphokines. The MChF‐producing T cell hybridomas reactive to specific antigens (PC) will be promising tools for analysis of molecular nature of MChF as well as molecular aspects of antigen recognition mechanism of T cells.