Androgen regulated expression of the alpha 2u-globulin gene in pancreatic hepatocytes of rat.

Open Access
Publisher Website
Google Scholar
Abstract
Under a copper-deficient regimen, pancreatic cells in the adult rat can be found to undergo differentiation into hepatocytes. Pancreatic hepatocytes induced in male and female rats were examined for the expression of the androgen-inducible hepatic protein, .alpha.2u-globulin. .alpha.2u-Globulin protein was demonstrable by immunoperoxidase method in all the pancreatic hepatocytes of male rats. Northern blot analysis confirmed the pesence of 1.3 kb .alpha.2u-globulin mRNA transcript in the pancreas of male rats with hepatocytes. Orchidectomy resulted in marked decrease of .alpha.2u-globulin protein and its mRNA. Administration of dihydrotestosterone to castrated rats resulted in increased levels of .alpha.2u-globulin mRNA and the amount of .alpha.2u-globulin protein in the pancreatic hepatocytes. Unlike normal males, in intact and ovariectomized females .alpha.2u-globulin was not detectable in pancreatic hepatocytes. These results indicate that similar to hepatic parenchymal cells pancreatic hepatocytes synthesize .alpha.2u-globulin under androgenic regulation. Furthermore, unlike in liver where it is expressed predominantly in perivenular and midlobular hepatocytes, there is no localized difference in the expression of this gene in the transdifferentiated pancreatic hepatocytes.