Studies on Peptides from a Peptic Hydrolyzate of Cytochrome c from Rhodospirillum rubrum.

Abstract
Cytochrome c (550 Rhodospirillum rubrum) was prepared by acid extraction, precipitation with (NH4) 2SO4 and TCA and chromatography on DEAE and CM[long dash]W. It was studied by spectrophotometry and electrophoresis. Two peptides were isolated by partition chromatography.after cytochrome-c digestion with pepsin. They have been subjected to amino acid analysis and N-terminal end group determination. The peptides were investigated by ultra-centrifugation and spectrophotometry.

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