ASSAY OF MALT β-GLUCANASE USING AZO-BARLEY GLUCAN: AN IMPROVED PRECIPITANT
Open Access
- 4 March 1987
- journal article
- research article
- Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing
- Vol. 93 (2) , 87-90
- https://doi.org/10.1002/j.2050-0416.1987.tb04481.x
Abstract
A procedure recently described for the assay of malt β-glucanase, which employs a dye-labelled and chemically-modified barley β-glucan substrate, has been improved by changing the precipitant solution used to terminate the reaction. The new precipitant solution contains 0·4% (w/v) zinc acetate and 4% (w/v) sodium acetate dissolved in 80% (v/v) aqueous methyl cellosolve. With this precipitant the procedure can be directly applied to the assay of cellulase activity, and with minor modification, to the assay of lichenase activity.Keywords
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