A Plant Peptide Encoded by CLV3 Identified by in Situ MALDI-TOF MS Analysis
Top Cited Papers
- 11 August 2006
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 313 (5788) , 845-848
- https://doi.org/10.1126/science.1128439
Abstract
The Arabidopsis CLAVATA3 ( CLV3 ) gene encodes a stem cell–specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI-TOF MS) applied to in situ Arabidopsis tissues determined the structure of a modified 12–amino acid peptide (MCLV3), which was derived from a conserved motif in the CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying the typical phenotype of transgenic plants overexpressing CLV3 . These results suggest that the functional peptide of CLV3 is MCLV3.Keywords
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