A Rapid and Time-Effective Assay for Determination of Oxolinic Acid and Flumequine in Fish Tissues by HPLC

Abstract
A simple method for analysis of oxolinic acid and flumequine in fish muscle and liver is described. The samples were extracted with trichloroacetic acid, neutralized and analysed by HPLC, with minimal sample manipulation. The limit of quantification was 30 μg/kg for oxolinic acid and 35μg/kg for flumequine. The simplified extraction and clean-up procedure makes it possible for one person to monitor the concentration of the drugs in approximately 80 samples per day.