CHARACTERIZATION OF ANTIGENS FROM ASPERGILLUS-FUMIGATUS .1. PREPARATION OF ANTIGENS FROM ORGANISMS GROWN IN COMPLETELY SYNTHETIC MEDIUM

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Abstract
A. fumigatus grew well in completely synthetic asparagine glycerol medium. Four day old mycelia were totally disrupted by vigorous mechanical shaking with 0.45-.mu.m glass beads. Such mechanically prodced extracts contained at least 52 precipitating antigens. The number of protein and carbohydrate components was maximal in 4 day old organisms and decreased in older cultures. The number of components in culture filtrates increased with longer incubation periods, but was only approximately 1/2 that produced by the 4 day old mycelial extracts.