The human rod photoreceptor cGMP phosphodiesterase β‐subunit
- 2 August 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 327 (3) , 275-278
- https://doi.org/10.1016/0014-5793(93)81003-i
Abstract
CDNA clones encoding the beta-subunit of the photoreceptor cGMP phosphodiesterase (PDE) were isolated from a human retina library and their sequence was determined. The encoded polypeptide consists of 854 amino acid residues with a calculated molecular mass of 98,416 Da. Alignment of the deduced amino acid sequence with the earlier analysed alpha-, beta- and alpha'-subunits of bovine and mouse PDEs demonstrates a high homology. Two overlapping recombinant lambda phage clones containing 26 kb of the human PDE beta-subunit gene were isolated from the genomic library. A total nucleotide sequence of exons 4-22 of the PDE beta-subunit gene was established which completely corresponded to the cDNA structure. According to sequence analysis no potential possibility for alternative splicing of the beta-subunit gene was observed between exons 20 and 21 which led to the formation of the beta'-subunit as described for mouse PDE. Polymerase chain reaction (PCR) experiments also confirm the absence of the PDE beta'-subunit in human retina.Keywords
This publication has 18 references indexed in Scilit:
- Molecular characterization of human and bovine rod photoreceptor cGMP phosphodiesterase α-subunit and chromosomal localization of the human geneGenomics, 1990
- Identification of a noncatalytic cGMP-binding domain conserved in both the cGMP-stimulated and photoreceptor cyclic nucleotide phosphodiesterases.Proceedings of the National Academy of Sciences, 1990
- Molecular model of the G protein alpha subunit based on the crystal structure of the HRAS protein.Proceedings of the National Academy of Sciences, 1989
- The three-dimensional structure of c-H-ras p21: implications for oncogene and G protein studiesTrends in Biochemical Sciences, 1988
- cGMP phosphodiesterase of retinal rods is regulated by two inhibitory subunits.Proceedings of the National Academy of Sciences, 1988
- Cyclic GMP phosphodiesterase from bovine retina Amino acid sequence of the α‐subunit and nucleotide sequence of the corresponding cDNAFEBS Letters, 1987
- Cyclic GMP phosphodiesterase from cattle retinaFEBS Letters, 1986
- Cyclic GMP Cascade of VisionAnnual Review of Neuroscience, 1986
- A simple and rapid method for sequencing DNAFEBS Letters, 1985
- TECHNOLOGICAL EXAMINATION OF LOW‐FIRED TERRACOTTA STATUES FROM AYIA IRINI, KEAArchaeometry, 1982