Tricaine (MS‐222) Induced Modification on the Metabolism of Foreign Compounds in the Liver and Duodenal Mucosa of the Splake (Salvelinus fontinalis X Salvelinus namaycush)

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Abstract
The splake, a popular game fish, is a crossbreed which must be reared in nurseries. The fish are marked under anaesthesia for later study. We analyzed the effect of a common anaesthetic, tricaine (MS-222), on the metabolism of foreign compounds in the liver and duodenum of the splake. In the liver and to some extent in the duodenum, aryl hydrocarbon hydroxylase and epoxide hydrase activities were reduced during treatments. The ethoxycoumarin O-deethylase activities were not affected in either the liver or duodenum. Tricaine significantly decreased the hepatic UDP-glucuronosyltransferase activity. The decrease was greater when the aglycone used was p-nitrophenol than with methylumbelliferone. A similar effect was also found after trypsin treatment of the microsomes. No significant decrease in the UDP-glucuronosyltransferase activity was detected in the duodenal mucosa. This was the case when both p-nitrophenol and methylumbelliferone were used as aglycones.