Biopsy specimen identification by detection of sex chromosomes: application of in situ hybridisation.
Open Access
- 1 February 1992
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 45 (2) , 149-150
- https://doi.org/10.1136/jcp.45.2.149
Abstract
AIMS: To investigate the feasibility of non-radioactive in situ hybridisation (ISH) for the identification of sex-mismatched plastic embedded bone marrow biopsy specimens. METHODS: After a suspected accidental transposition of two glycol-methacrylate embedded bone marrow specimens, in situ hybridisation with sex chromosome specific probes was performed. RESULTS: Quantitative analysis of the hybridisation signals established unequivocably the origin of the specimens. CONCLUSIONS: ISH is feasible on GMA embedded bone marrow specimens, and can be used for the identification of accidentally transposed specimens provided that they are of sex-matched origin.Keywords
This publication has 8 references indexed in Scilit:
- Detection of trisomy 8 in hematological disorders by in situ hybridizationCytogenetic and Genome Research, 1991
- HUMAN PAPILLOMAVIRUS DNA IN RESPIRATORY PAPILLOMATOSIS DETECTED BY INSITU HYBRIDIZATION AND THE POLYMERASE CHAIN-REACTION1989
- SUPERNUMERARY CHROMOSOME-1 IN INTERPHASE NUCLEI OF ATYPICAL GERM-CELLS IN PARAFFIN-EMBEDDED HUMAN SEMINIFEROUS TUBULES1989
- INTERPHASE CYTOGENETICS IN PARAFFIN EMBEDDED SECTIONS FROM HUMAN TESTICULAR GERM-CELL TUMOR XENOGRAFTS AND IN CORRESPONDING CULTURED-CELLS1989
- DETECTION OF CHROMOSOME ANEUPLOIDY IN INTERPHASE NUCLEI FROM HUMAN PRIMARY BREAST-TUMORS USING CHROMOSOME-SPECIFIC REPETITIVE DNA PROBES1988
- Isolation and characterization of a major tandem repeat family from the human X chromosomeNucleic Acids Research, 1983
- Characterisation of a human Y chromosome repeated sequence and related sequences in higher primatesChromosoma, 1982
- Methyl‐methacrylate as an embedding medium in histopathologyHistopathology, 1977