Systemic Spread ofAgrobacterium tumefaciensBiovar 3 in the Vascular System of Grapes

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Abstract
The systemic movement of Agrobacterium tumefaciens biovar 3 was monitored with a mutant that was resistant to both streptomycin and rifampicin. Movements during the course of the experiments was confined exclusively to the xylem vessels. The bacteria moved rapidly 30 cm from the base of cut grape shoots. Additional evidence for long-distance vascular movement of A. tumefaciens was the development of tumors on aseptically wounded stem tissue 24 cm from the site of inoculated root tissue.