Radiochemistry and biostability of autologous leucocytes labelled with 99mTc-stannous colloid in whole blood
- 30 April 1984
- journal article
- research article
- Published by Springer Nature in European Journal of Nuclear Medicine and Molecular Imaging
- Vol. 9 (5) , 216-219
- https://doi.org/10.1007/bf00448542
Abstract
Autologous leucocytes were labelled in whole blood by phagocytic uptake of 99mTc-stannous colloid. This colloid has a mean particle size of 1.5 μm and labels leucocytes with 81% efficiency. Individual cell uptakes were: granulocytes 42%, monocytes 39%. Isotonic sodium citrate added after the labelling procedure did solubilise excess colloid but was not necessary for adequate removal of excess colloid. Labelled leucocytes were shown in vitro to be viable and maintain normal bactericidal and chemotactic capacity. Biodistribution, clearance rates and dosimetry are presented. These results indicate that autologous leucocytes can be efficiently labelled with 99mTc-stannous colloid with good residual cell function.Keywords
This publication has 7 references indexed in Scilit:
- ORGAN DISTRIBUTION OF CANINE LEUKOCYTES LABELED WITH TC-99M-SULFUR COLLOID1977
- IN-111-LABELED LEUKOCYTES FOR LOCALIZATION OF ABSCESSES - PREPARATION, ANALYSIS, TISSUE DISTRIBUTION, AND COMPARISON WITH GA-67 CITRATE IN DOGS1977
- SURVEY OF RADIOACTIVE AGENTS FOR INVITRO LABELING OF PHAGOCYTIC LEUKOCYTES .2. PARTICLES1976
- Separation of Blood Leucocytes, Granulocytes and LymphocytesTissue Antigens, 1974