Adaptation of Respiratory Syncytial (RS) Virus to Brain of Suckling Mice

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Abstract
Summary Respiratory syncytial virus (Long strain) was adapted to growth in suckling mice through 2 separate serial intracerebral passage series. The virus(es) produced a lethal effect in the animals 6 to 14 days following inoculation. Identification of the mouse passage virus was established by serum neutralization tests in tissue culture and in suckling mice. Further identification was established by direct fluorescent antibody staining of the mouse passaged virus after one passage in tissue culture. It is suggested that the growth of RS virus in brain of suckling mouse with resultant illness and death may have practical value in the evaluation of prospective vaccines.