RAPID SEPARATION OF ENKEPHALINS AND ENDORPHINS ON SEP‐PAK® REVERSE PHASE CARTRIDGES
- 1 July 1981
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 18 (1) , 107-110
- https://doi.org/10.1111/j.1399-3011.1981.tb02045.x
Abstract
A simple, rapid method is described for separating neuropeptides. Samples were injected on Sep-Pak® disposable reverse phase cartridges and eluted with a step gradient of n-propanol in formate-pyridine buffer. Enkephalins are separated from metabolic products. Separation of α, β and γ-endorphin is demonstrated, and Met- and Leu-enkephalin are also separated. This inexpensive, reproducible method is useful in studies of neuropeptide metabolism, purification of synthesized peptides, separation prior to RIA, or concentration of neuropeptides.Keywords
This publication has 7 references indexed in Scilit:
- SEPARATION OF OPIOID PEPTIDES UTILIZING HIGH PERFORMANCE LIQUID CHROMATOGRAPHYInternational Journal of Peptide and Protein Research, 2009
- DEVELOPMENT OF A SPECIFIC EXTRACTED RADIOIMMUNOASSAY FOR METHIONINE ENKEPHALIN IN HUMAN PLASMA AND CEREBROSPINAL FLUIDJournal of Endocrinology, 1980
- Cleavage of endorphins to des-Tyr endorphins by homogeneous bovine brain aminopeptidaseNature, 1980
- A Rapid and Specific Method for the High Resolution Purification and Characterization of NeuropeptidesJournal of Neurochemistry, 1980
- A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissuesBiochemical Journal, 1978
- Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Application to the identification of circulating metabolites of corticotropin-(1-24)-tetracosapeptide and somatostatin in vivoBiochemical Journal, 1977
- Mode of deactivation of the enkephalins by rat and human plasma and rat brain homogenatesNature, 1976