ESTABLISHMENT AND CHARACTERIZATION OF 3 NEW CONTINUOUS CELL LINES DERIVED FROM HUMAN BREAST CARCINOMAS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (10) , 3352-3364
Abstract
Three continuous lines of mammary tumor cells (ZR-75-1, ZR-75-27 and ZR-75-30) were established from malignant effusions of 2 women with breast cancer. Differentiated properties expressed by each cell line include: epithelial morphology (by light microscopy and EM) resembling that of the parental tumors; presence of receptors for estrogen and other steroid hormones and growth responsiveness to estrogen and/or progesterone. All 3 cell lines possess human karyotypes that differ from one another in modal chromosome number as well as in characteristic marker chromosomes. Two of the cultures (ZR-75-27 and ZR-75-30), although derived from the same patient, have stable differences in their karyotypes.